WebChromogenic in-situ hybridization (CISH) is a relatively new method for detection of gene amplification using a peroxidase reaction, which can be viewed using a standard light microscope. This study was undertaken to validate CISH as a method for assessing human epidermal growth factor receptor-2 gene amplification. WebThe testing lab typically uses a specialized staining process on the breast tissue sample to see if hormone receptors are present. The technical …
CISH - an overview ScienceDirect Topics
WebMar 29, 2024 · CISH cytokine inducible SH2 containing protein Gene ID: 1154, updated on 8-Dec-2024 Gene type: protein coding Also known as: CIS; G18; SOCS; CIS-1; BACTS2 … WebCISH Purpose of the test Help This is a clinical test intended for Help: Diagnosis, Mutation Confirmation Condition Help 3 conditions tested. Click Indication tab for more … smallpox montreal
Chromogenic in-situ hybridization: a viable alternative to
Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for … See more Probe design Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags … See more Compared to FISH FISH is considered to be the gold standard for the detection of chromosomal abnormalities because it is very sensitive and has high … See more Silver-enhanced in situ hybridization (SISH) SISH uses a similar method as CISH, but a silver precipitate is the end product rather than a brown product. In this method, a probe tagged with dinitrophenol (DNP) binds … See more CISH is frequently applied to assess gene amplification, such as HER-2/neu status in breast cancer samples. HER-2/neu amplification is … See more WebFluorescence in situ Hybridization (FISH), Oncology TEST: 510669 CPT: Contact CPT coding department at 800-222-7566, ext 6-8400. Print Share Include LOINC® in print Special Instructions Call for additional probes not listed above. WebThe HER2 and Chromosome 17 probes are detected using two-color chromogenic in situ hybridization (ISH) in formalin-fixed, paraffin-embedded human breast and gastric carcinoma tissue specimens, … smallpox medication