Dethiobiotin synthase

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WebMar 3, 1998 · HPLC analysis of reaction mixtures revealed the presence of a compound with the characteristics of an intermediate: (1) it was labelled with 14C, and therefore derived from the [14C]dethiobiotin substrate; (2) it was present only in reaction mixtures containing biotin synthase; (3) it was not derived from [14C]biotin; (4) 35S from [35S]cystine ... Biotin synthase (BioB) (EC 2.8.1.6) is an enzyme that catalyzes the conversion of dethiobiotin (DTB) to biotin; this is the final step in the biotin biosynthetic pathway. Biotin, also known as vitamin B7, is a cofactor used in carboxylation, decarboxylation, and transcarboxylation reactions in many organisms including humans. Biotin synthase is an S-Adenosylmethionine (SAM) dependent enzym…

Dethiobiotin synthase - Wikipedia

WebKrell K, Eisenberg MA: The purification and properties of dethiobiotin synthetase. J Biol Chem. 1970 Dec 25;245(24):6558-66. Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR: Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties. WebOct 15, 1998 · The crystal structure of the 224-residue protein dethiobiotin synthetase from Escherichia coli has been refined using X-ray diffraction data at 0.97 A resolution at 100 K. The model, consisting of 4143 protein atoms including 1859 H atoms and 436 solvent sites, was refined to a final R factor of 11.6% for all reflections, and has an estimated ... t shirts 4u

Biotin Synthase - an overview ScienceDirect Topics

WebDec 14, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. … WebBiotin synthase (BioB) catalyzes the insertion of a sulfur atom between the C6 and C9 carbons of dethiobiotin. Reconstituted BioB from Escherichia coli contains a [4Fe-4S]2+/1+ cluster thought to be involved in the reduction and cleavage of S-adenosylmethionine (AdoMet), generating methionine and the reactive 5′-deoxyadenosyl radical responsible … WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. … t-shirts 4 u

RCSB PDB - 1DTS: CRYSTAL STRUCTURE OF AN ATP DEPENDENT …

1245251 - Gene ResultbioD dethiobiotin synthase []

WebGSE12663: virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages: GSE42863: Flagellar export apparatus protein, FliH, is involved in post-transcriptional regulation of FlaB, motility and virulence of the relapsing fever spirochete Borrelia hermsii WebDethiobiotin synthetase Methods Enzymol. 1979;62:348-52. doi: 10.1016/0076-6879 (79)62241-3. Authors M A Eisenberg , K Krell PMID: 374977 DOI: 10.1016/0076-6879 … t-shirts 4xl sizeWebMar 28, 1995 · One of these enzymes, ATP-dependent dethiobiotin synthetase, catalyzes the carboxylation of 7,8-diaminopelargonic acid leading to the formation of the ureido ring of biotin. The enzyme belongs to the class of ATP-dependent carboxylases and we present here the first crystal structure determined for this class of enzyme. t-shirts 4u s.r.o

"WebJul 1, 2007 · KAPA synthase from A. thaliana was localized in the cytosol by confocal microscopy of the GFP-fused enzyme and by Western blotting (Pinon et al., 2005). Sequence homologs of the bacterial 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin synthase exist in the A. thaliana genome (Pinon et al., 2005). " - Dethiobiotin synthase

Dethiobiotin synthase

WebMar 9, 2024 · biotin synthase. GeneRIFs: Gene References Into Functions. In biotin synthase, abstraction of a hydrogen atom from the C9 methyl group of dethiobiotin results in formation of a dethiobiotinyl methylene carbon radical, which is quenched by a sulfur atom to form a new carbon-sulfur bond in 9-mercaptodethiobiotin. WebNov 18, 2005 · Working model for the compartmentation of biotin metabolism in the plant cell. Biotin-synthesizing enzymes are as follows: 1, KAPA synthase; 2, DAPA aminotransferase; 3, dethiobiotin synthase; and 4, biotin synthase. The occurrence of reaction 2 in the cytosol and of reaction 3 in mitochondria is predicted from DNA …

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WebThis article is published in Journal of the American Chemical Society.The article was published on 1952-06-01. It has received 43 citation(s) till now. WebDethiobiotin synthase is the penultimate enzyme in the biotin synthesis pathway in E. coli and other microorganisms. The enzyme catalyses the formation of the ureido ring …

Webdethiobiotin synthase. DTB synthetase. (7R,8S)-7,8-diammoniononanoate + ATP + CO2 <=> (4R,5S)-dethiobiotin + ADP + 3 H (+) + phosphate. CTP has half the activity of …

WebApr 7, 1999 · Biotin synthase, the enzyme which catalyses the conversion of dethiobiotin (DTB) to biotin has an absolute requirement for S-adenosylmethionine (AdoMet) which is … WebDethiobiotin synthase is involved in biotin biosynthesis and catalyses the reaction (CO2 + 7,8-diaminononanoate + ATP = dethiobiotin + phosphate + ADP). The enzyme binds ATP (see motif in first 12 residues of the SEED alignment) and requires magnesium as a co-factor. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin]

WebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a …

WebAug 21, 2012 · DAPA synthase and dethiobiotin synthase are encoded by adjacent genes that can be alternatively spliced to specify a bifunctional fusion protein or separate enzymes (Muralla et al., 2008). The fusion and the separate dethiobiotin synthase are predicted to be mitochondrial and the separate DAPA synthase is predicted to be cytosolic; … t-shirts 4xl extra longWebDethiobiotin. The conversion of dethiobiotin (4) into biotin (5), which is catalyzed by biotin synthase, is the most novel step in biotin biosynthesis since it involves the formal … t-shirts 5 for 10WebEC Tree. 6 Ligases. 6.3 Forming carbon-nitrogen bonds. 6.3.3 Cyclo-ligases. 6.3.3.3 dethiobiotin synthase. IUBMB Comments. CTP has half the activity of ATP. Specify your search results. Mark a special word or phrase in this record: t shirts 50% cotton 50% polyesterIn enzymology, a dethiobiotin synthase (EC 6.3.3.3) is an enzyme that catalyzes the chemical reaction ATP + 7,8-diaminononanoate + CO2 ADP + phosphate + dethiobiotin The 3 substrates of this enzyme are ATP, 7,8-diaminononanoate, and CO2, whereas its 3 products are ADP, phosphate, and dethiobiotin. philosophy\\u0027s gxWebJan 11, 2024 · BioD, dethiobiotin synthase In contrast to the preceding enzymes (with the exception of BioU), BioD (DTB synthase) catalyzes an unusually interesting step. This is the mechanistically unusual reaction of ATP (or CTP) dependent insertion of CO 2 between the N7 and N8 nitrogen atoms of DAPA to form the ureido moiety of biotin. philosophy\\u0027s gwWebdethiobiotin synthase. Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer. bioD dethiobiotin synthase [] Gene ID: 1245251, discontinued on 29-Dec-2024. Summary. Other designations. dethiobiotin synthase ... philosophy\u0027s gwWebJun 13, 2005 · Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. Gene Name bioB Uniprot … philosophy\\u0027s gy