WebClone TRA-1-60. Analysis of TRA-1-60 Antigen in H9 cells. H9 human embryonic stem (ES) cells were stained with either FITC Mouse Anti-Human TRA-1-60 Antigen (solid line) or FITC Mouse IgM, κ Isotype Control (clone G155-228, Cat. No. 553474, dashed line), incubated in the dark for 20 minutes at room temperature and analyzed by flow cytometry. WebFeb 10, 2024 · Py 3-FITC can be used to detect TGF-β1-induced collagen type I. HFL-1 cells were treated with TGF-β1 for 0 h (a–c) or 48 h (d–f) and then stained with Py 3-FITC (a,d) and collagen type I antibody (b,e), and their merged images are shown (c,f). Collagen type I expression was increased by 48 h of TGF-β1 treatment.
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Web1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. WebDrive revenue growth and maximize business opportunities. Accept and process cards, PayPal, and wallets such as Venmo (US only), Apple Pay, and Google Pay as well as enable buy now, pay later options in a single, … first oriental market winter haven menu
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WebFITC conjugates are not very stable to elevated temperatures and are very pH sensitive (in terms of their fluoresence behavior; fluoresence yield was found to be higher at pH 7.4 than at pH 6.0 by ... WebThe FITC solution is then diluted (1:50, 1.5:100, 1:100, 1:200) with PBS buffer by the students. 2.2. Bioconjugation. The protocol used for the modification of BSA with FITC is reported in literature 1 and is very simple: 1) Students are asked to prepare a 2 mg/ml BSA solution in 0.1 M sodium carbonate, pH 9. WebFit Factory Braintree is a full-service health club located in Massachusetts. Call 781-277-0402 for more information about working out at our gym. YOUR FIRST WORKOUT IS … first osage baptist church