Nitrate reductase clone and expression

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August undefined, 2024

WebbThe accumulation of both leaf and root NiR mRNAs was induced by nitrate and repressed by nitrate- or ammonium-derived metabolites. In addition, the expression of the root NiR gene was detectable in leaves of a tobacco nitrate reductase (NR)-deficient mutant. WebbThree tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. Sequence analysis and Southern blot hybridization revealed four …

Isolation, expression, and biochemical characterization: nitrite ...

Webb13 apr. 2024 · Expression of the following genes related to nitrogen metabolism was determined: NR genes OsNia1 (LOC4345795) and OsNia2 (LOC4330867), nitrate … WebbThe nitrate transporter polypeptide (NRT2) is characterised by 12 transmembrane domains and presents both a long putative intracellular loop and a short C-terminal … toilettentraining plan

Characterisation and expression analysis of a nitrate ... - Springer

WebbThe molecular mass of the encoded protein was predicted to be 99.5 kDa, with an isoelectric point of 8.31. This protein shares common structural features with NRs from … Webb12 maj 2011 · Energy supply by a carbon source seems to be the major regulator of nitrate reductase gene expression for fungi ... soils for >50% of the clones. Fungal euknr gene expression in ... toilet tent camping

Cloning and expression of distinct nitrite reductases in tobacco …

Cloning and expression of distinct nitrite reductases in ... - Springer

Webb15 juni 2015 · The nitrate reductase gene (nir), encoding the nitrite reductase enzyme (NiR) in Lactobacillus plantarum DMDL 9010, was cloned, and the expressed product was purified. The nir fragment was... Webb1 jan. 1999 · NADH-Cyt b5 reductase is a membrane-bound flavoprotein containing a single FAD as a prosthetic group. It transfers electrons from NADH to Cyt b5, which is another membrane protein containing a single heme group. toilettensitz villeroy und boch subwayWebb31 mars 2024 · Gene Expression. nirA, ... nirA d-106 9 an allele conferring nitrogen metabolite derepression of nitrate and nitrite reductase activity, includes two transitions, changing a glutamic acid to a lysine and a valine to an alanine, situated between a basic and a proline-rich region of the protein. toilettentrainer wal

"WebbCloning and expression of distinct nitrite reductases in tobacco leaves and roots. Three tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR … " - Nitrate reductase clone and expression

Nitrate reductase clone and expression

Cloning, expression, and purification of the nitrite reductase …

Webb15 nov. 2007 · The purpose of this work was to investigate whether heterologous gene expression in transgenic Dunaliella salina would be controlled by an inducible promoter. Here we identify that the nitrate reductase (NR) transcripts of D. salina are induced by nitrate but repressed by ammonium. WebbAll reductive branches of this cycle involve the conversion of nitrate to nitrite, which is catalyzed by the enzyme nitrate reductase. The characterization of nitrate reductases …

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WebbA partial cDNA clone for barley nitrate reductase has been isolated and identified by hybrid-selected translation. RNA blot-hybridization analysis shows that nitrate induction also causes a marked increase in the steady-state level of nitrate reductase mRNA. Continue Reading VIEW PDF Download PDF Further reading in this issue [...] Webb1 juni 1992 · cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD (P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark. Gowri G , Campbell WH Plant Physiol, (3):792-798 1989 MED: 16666879

Webb28 jan. 2010 · Nitrate reductase is a high-molecular-weight complex protein with three prosthetic groups, FAD, heme (cytochrome b 557 ), and Mo-pterin, which uses NADH … WebbKeywords Cloning.Dunaliella salina.Chlorophyta. Nitratereductase.Overexpression Introduction Nitrate is the major compound for nitrogen acquisition in nature. In most …

Webb1 nov. 2003 · Expression of YNR1 under control of the MOX promoter in the presence of ammonium or nitrate. H. polymorpha strain bearing the YNR1 -coding region fused to the MOX gene promoter was grown in ammonium-containing medium. WebbThe term dissimilatory N-oxide reduction is also used to indicate that the products are lost to the environment; this distinguishes the reactions from the assimilatory reductions used by plants and bacteria to incorporate nitrogen into biomass. 1.1.1The importance of …

Webb13 apr. 2024 · Our study shows that expression of NPF8.4 and most ... glycolate oxidase; HPR1, hydroxypyruvate reductase 1; 2OG, 2 ... Table 4), and cloned into the oocyte expression vector ...

Webb29 mars 2003 · Further work has led to the selection of nitrate reductase-deficient mutants (Marmeisse et al. 1998) and the cloning of a functional nitrate reductase encoding gene (nar1; Jargeat et al. 2000). Transcription of this gene is strongly but not fully repressed by ammonium and, surprisingly, does not need nitrate for induction, as … peoplestrong login evryWebbThe nitrate reductase of fungi and plants is a soluble (or easily solubilized) NADPH-dependent assimilatory enzyme. Bacteria possess either a soluble assimilatory enzyme … toilettentrainer costwayWebb23 dec. 2014 · Nitrite reductase (NiR) is a critical enzyme involved in nitrogen metabolism in plants. A full-length cDNA sequence of 1,764 bp for the NiR gene was isolated from … peoplestrong login integraWebbNitrate is the major source of nitrogen taken from the soil by higher plants but requires reduction to ammonia prior to incorporation into amino acids. The first … peoplestrong login home firstWebb6 okt. 2024 · Expression of MRA 2164 and MRA0854 gene in Mycobacterium tuberculosis The expression of the possible nitrite reductase genes ( MRA2164 and MRA0854) were checked by estimating their respective... toiletten stand wcWebbNitrate reductase is a key enzyme in nitrogen assimilation, and it catalyzes the nitrate-to-nitrite reduction process in plants. A variety of factors, including nitrate, light, … peoplestrong login netenrichWebbCloning and expression of distinct nitrite reductases in tobacco leaves and roots J. Kronenberger, A. Lépingle, +1 author H. Vaucheret Published 2004 Biology, Medicine Molecular and General Genetics MGG SummaryThree tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. peoplestrong login qualitykiosk